Download Advances in Biochemical Engineering Biotechnology by Wolfgang Babel, Alexander Steinbüchel PDF

By Wolfgang Babel, Alexander Steinbüchel

Dwelling structures synthesize seven assorted periods of polymers. they supply constitution and shape for cells and organisms, functionality as catalysts and effort garage and hold the genetic details. these types of polymers own technically fascinating homes. a few of these biopolymers are already used commercially. This designated quantity of Advances in Biochemical Engineering/Biotechnology contains 10 chapters. It offers an outline of the water insoluble biopolyesters, particularly of the microbially synthesized poly-hydroxyalkanoate (PHA) kinfolk. It studies the cutting-edge of metabolism, rules and genetic history, the most recent advances made in genetic optimization of micro organism, "construction" of transgenic vegetation and in vitro synthesis through purified enzymes. additionally, it describes appropriate applied sciences and evaluates views bearing on expanding the industrial viability and competitiveness of PHA and discusses purposes in drugs, packaging, meals and different fields.

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126. 127. 128. 47 Sherf B, Bajar AM, Kolattukudy PE (1993) Plant Physiol 101:201 Hankin L, Kolattukudy PE (1971) Plant Soil 34:525 Patty BR, Chandra AK (1981) Plant Soil 61:419 Sebastian J, Chandra AK, Kolattukudy PE (1987) J Bacteriol 169:131 Sebastian J, Kolattukudy PE (1988) Arch Biochem Biophys 263:77 Sebastian J (1987) Discovery and purification of cutinase and cloning of cutinase gene from a Pseudomonas sp. cohabitting with an apparently nitrogen-fixing Corynebacterium sp. in the phyllosphere.

Solani f. pisi and sequenced (T. E. Kolattukudy, unpublished). Cut1 is the inducible gene that requires CTF1a. Cut2 and cut3 have two nucleotide substitutions in palindrome 1 whereas palindrome 2 is identical to that of cut1. The two nucleotide substitution pre- Polyesters in Higher Plants 41 Fig. 13. Postulated mechanism by which cutin monomers produced by constitutive expres- sion of cut2/3 induce cut1 vents palindrome 1 of cut2/cut3 from binding PBP as can be demonstrated by gel retardation analysis.

The evidence that suggests that cutinase may be important in this process can be summarized as follows [27]: (a) Pathogenic fungi produce and secrete cutinase targeted at the penetration point and during actual infection of the host such an enzyme is produced as detected immunocytochemically. (b) Specific inhibition of cutinase by chemicals or antibodies including monoclonal antibodies prevents infection. (c) Cutinase-deficient mutants have significantly reduced virulence but infection can be restored with exogenous cutinase.

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