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By Hasegawa F., Hashima M., Kanda Sh.

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This means that movement of Na+ in solution is caused by the relative concentration of Na+ alone and is not affected by the concentration of other ions in solution. 2 mV (ENa+). Alternatively late outward currents would be expected to be carried by K+ with a Erev more negative than -60 mV. 14 mV) clearly indicated that currents were passing through the point of origin demonstrating non-selective ion permeation. The single-channel current responses noted for cells in physiologically normal solutions indicated the probability of multiple channel subconductance states (Figure#21).

This result was also confirmed by other groups (Donnelly-Roberts et al, 2004; Faria et al, 2010; Lundy et al, 2004). However, Roger and coworkers (Roger et al, 2008, 2010) reported that rat P2X7R induced large organic cation permeability ionic currents were dependent on critical residues of calmodulin binding domain when recorded in patch-clamp whole cell configuration. In this sense, the intracellular Ca2+ concentration dependence in the P2X7R pore formation is still unclear. We have shown in 2005 that intracellular Ca2+ acts as a second messenger in the large conductance channel opening in peritoneal macrophages and 2BH4 cells (Faria et al, 2005).

14. Whole-Cell Current Response of the High B103 Subset to Transient Bath Application of 500 M 5-HT in Symmetrical Solutions. 5 2 1 (A) Demonstrates a typical oscilloscope diagram of the whole-cell current response to the applied  indicates an entire Protocol response,  the maximum (+30 mV) depolarising response, and  the maximum (-70 mV) hyperpolarising response. (B) Shows the current response (pA) versus applied voltage (mV) plot for the whole-cell recorded data in experimental solutions.

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